Mouse Syndecan-1/SDC1 PicoKine ELISA Kit

  • Catalog number
    EK1554
  • Price
    Please ask
  • Size
    1x96 well plate
  • ELISA s application
    For quantitative measurement of mouse Syndecan-1 in cell culture supernatant , cell lysate, tissue homogenates, serum and plasma heparin, EDTA .
  • Gene tested
    murine Syndecan-1
  • ELISA standard curve
    Concentration(pg/ml) + 0 + 156 + 312 + 625 + 1250 + 2500 + 5000 + 10000 O.D. + 0.002 + 0.083 + 0.147 + 0.314 + 0.636 + 1.159 + 1.802 + 2.258
  • ELISA kit detection limits
    156pg/ml to 10000pg/ml
  • ELISA s sensitivity
    < 10pg/ml
  • Target of this ELISA
    Natural and recombinant mouse Syndecan-1
  • Cross reaction
    the ELISA does not cross react with any other proteins except from closely related species
  • ELISA s precision parameters
    intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);223;795;3254;196;814;4109 ELISA standard deviations 11.37;42.13;165.95;7.64;34.18;209.55 CV(%);5.1;5.3;5.1;3.9;4.2;5.1
  • ELISA kit description
    GENTAUR's mouse Syndecan-1 ELISA test is a ready to use ELISA kit. We use a monoclonal from rat specific for Syndecan-1 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: Q18-E252) and your samples are measured with a biotin conjugated polyclonal from goat specific for Syndecan-1 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more mouse Syndecan-1 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
  • Detector and capure antibodies
    A rat monoclonal detector antibodies is used with a goat biotin polyconal capture antibodies.
  • The ELISA contains
    Catalog number;Description;Quantity -;96-well plate precoated with anti- mouse Syndecan-1 antibodies;1 ST0000-10;Lyophilized recombinant mouse Syndecan-1 standard;10ng/tube×2 AR1107;Biotinylated anti- mouse Syndecan-1 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
  • Anti coagulation
    heparin or EDTA
  • How the elisa works
    Sample the amount of 100ul/well of the standard solution at 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml mouse Syndecan-1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatant, cell lysate, tissue homogenates, serum or plasma (heparin, EDTA) to the ELISA plate. Keep in mind that each mouse Syndecan-1 standard solution and each sample be measured in duplicate.
  • Minutes of TMB incubation
    25-30
  • More gene info
    Syndecan 1, also known as SYND1 or CD138, is a protein which in humans is encoded by the SDC1 gene. The protein encoded by this gene is a transmembrane(type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. This gene is mapped to 2p24.1. The syndecans mediate cell binding, cell signaling, cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan 1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. What’s more, Syndecan-1 is also a sponge for growth factors, with binding largely via heparan sulfate chains.
  • Antigen
    NSO,Q18-E252
  • Keep at
    Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
  • Uniprot
    Syndecan-1
  • Gene name
    syndecan 1
  • Alternative
    CD138 ; P18827 ; SDC1 ; SYND1 ; Synd-1 ; Synd1 ; syndecan1 ; Syndecan-1
  • Gen protein identity
    P18828
  • Entrez nr
    20969
  • Properties
    E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
  • Test
    ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED, Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
  • Gene symbol
    Sdc1
  • Short name
    Mouse Syndecan-1/SDC1 PicoKine ELISA Kit
  • Technique
    ELISA kit, ELISA, Mouse, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests. mouses
  • Host
    mouse, antigen assay ELISA kit 1 Kit (96 Wells)
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse Syndecan-1/SDC1 PicoKine Enzyme-linked immunosorbent assay reagent
  • Alternative technique
    murine, elisas, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
  • Tree numbers
    • E05.478.566.350.170
    • E05.478.566.380.360
    • E05.478.583.400.170
    • E05.601.470.350.170
    • E05.601.470.380.360
  • Qualifiers
    ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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