Human IgE-Biotin (myeloma, kappa), purified (isotype control, >95%, low endotoxin, azide free)

• Catalog number
  20007-3-LE-BTN
• Price
  Please ask
• Size
  100 ul

• Stock availability
  Available
• Category
  Isotype Control
• Antibody type
  N/A
• Antibody host
  N/A
• Antibody conjugate
  Biotin
• Technical datasheet
  Contact Gentaur to request the datasheet or ask our specialists for more information.
• Notes
  The Human IgE-Biotin (myeloma, kappa), purified (isotype control, >95%, low endotoxin, azide free) is manufactured for Research Use Only or for diagnostics purposes.
• Other related products
• Description
  Sodium azide NaN3 is often used to prevent antibody degradation in 0,02% W/V concentrations. adi supplies azide free antibodies on request. Recently adi made this IgE- (myeloma, kappa), purified (isotype control, >95%, endotoxin, ) available without azide. It will stick less and is more suitable for ELISA or in vivo studies. However you need to store the @VV@ frozen and ship on dry ice. Isotype or positive controls by peptides, antibodies and deactivated samples.
• Properties
  Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples. Immunoglobulin E (IgE) kappa or Fc specific antibody is a kind of antibody (or immunoglobulin (Ig) "isotype") that has only been found in mammals. IgE is synthesized by plasma cells. Monomers of IgE consist of two heavy chains (ε chain) and two light chains, with the ε chain containing 4 Ig-like constant domains (Cε1-Cε4). IgEs play a role in allergy and response to parasite infection. High levels of IgEs caused by parasites can lower the allergic reaction of patients to allergens common present in the human environment. As such the parasite history of an allergic patient needs to be taken in consideration as a positive factor. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
• Conjugation
  Biotinylated
• Group
  isotypes

• Gene target
  myeloma   kappa   endotoxin  
• Short name
  IgE- (myeloma, kappa), purified (isotype control, >95%, endotoxin, )
• Technique
  purified, Control, Isotype, IgE, controls, IgEs, Isotypes and controls, purifications
• Isotype
  IgE, IgE
• Label
  Biotin
• Species
  Human, Humans
• Alternative name
  H. sapiens immunoglobuline E-biotinilated (myeloma, kappa), enriched (isoform reference, >95%, low endotoxin, azide free)
• Alternative technique
  pure, control, controls, immunoglobulin-e
• Tissue
  control

MeSH Data

• Name
  Limulus Test
• Concept
  Scope note: Sensitive method for detection of bacterial endotoxins and endotoxin-like substances that depends on the in vitro gelation of Limulus amebocyte lysate (LAL), prepared from the circulating blood (amebocytes) of the horseshoe crab, by the endotoxin or related compound. Used for detection of endotoxin in body fluids and parenteral pharmaceuticals.
• Tree numbers
  • E01.370.225.124.470
  • E01.370.225.875.150.570
  • E05.091.570
  • E05.200.124.470
  • E05.200.875.150.570
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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