This antibody has been succesfully tested for use in following methods: Western blot
Target of this antibody: PRMT1
Formulation of this antibody: 100 µl affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA and 0.02% thimerosal.
Alternative names of antibody target: PRMT1, PRMT-1, Protein Arginine N-Methyltransferase 1, HRMT1L2, HMT1 hnRNP Methyltransferase-like 2, ANM1, Arginine N-Methyltransferase 1, IR1B4, Interferon Receptor 1-Bound Protein 4, HCP
Recommended usage: Western blotting (1:500 – 1:2500). However, the optimal concentrations should be determined individually. HeLa cell lysate can be used as a positive control. The antibody recognizes the PRMT1 of human and mouse origins. Reactivity to other species has not been tested.
Storage information: Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions: gel pack
Antibody host: Rabbit
This antibody reacts with: Human, mouse
Clonality of antibody: Polyclonal
Isotype: Rabbit IgG
Purification method: Affinity purified
Immunogene sequence: Synthetic peptide of human PRMT1 between amino acid 300 and the C terminus
How to handle this antibody: The antibody solution should be gently mixed before use.
Background information: PRMT1 methylate’s (mono & asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in a glycine and arginine-rich domain (may methylate HNRNPA1 and histones) methylate’s SUPT5H. The PRMT1 protein functions as a histone methyltransferase specific for H4. PRMT1 is an essential factor in oncogenesis and is a potential novel therapeutic target in cancer. PRMT1-mediated methylation serves as a positive modulator of IR/IRS-1/PI3K pathway and glucose uptake in skeletal muscle cells. CAF1 is a new regulator of PRMT1-dependent arginine methylation. PRMT1 arginine-methylate’s MRE11 therefore it regulates the activity of MRE11-RAD50-NBS1 complex during the intra-S-phase DNA damage checkpoint response. PRMT1 plays a post-translationally part in regulating the transcriptional activity. PRMT1 is found predominantly in the cytoplasm, though a fraction of PRMT1 is located in the nucleus.
Form of antibody: Liquid
Concentration of antibody: N/A
Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation anticorps