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We send this reagant on
Room temperature
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The temperatureerature for storage is
Upon receipt, aliquot and store the Nitrated BSA Standard at -20ºC to avoid multiple freeze/thaw cycles. Store all other test kit components at 4ºC.
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Technical file
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SDS or safty data sheet
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Type of reagent
Oxidative Stress/Damage
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Related techniques
Oxidative Stress/ Damage, Protein Oxidation / Nitration, Protein Nitration Assays and Reagents
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Use of the reagent
Nitric oxide influences a variety of biological processes including cell proliferation, apoptosis, neurotoxicity and extracellular matrix remodeling. Nitric oxide reacts with superoxide to form peroxynitrite, which in turn nitrates tyrosine residues in proteins. Nitrotyrosine therefore serves as a marker for peroxynitrite action in a variety of disease states and in conditions of cellular damage and oxidative stress. Our OxiSelect™ Nitrotyrosine ELISA test kit provides a sensitive method to measure the formation of 3-nitrotyrosine in proteins.
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Cellular use
Sensitive detection of 3-Nitrotyrosine as low as 10 nM
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Protocol information
Suitable for use with cell lysates, serum, plasma and purified proteins
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Reagent properties
Nitrated BSA provided as positive control
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Living modified organisme in this reagent or LMO
Not applicable
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Species compatible to
Humans
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Use of this test kit or product
Genetics lab
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Antigen
a specific protein sequence
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Buffer
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,27
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Molecular weight
in kilo Danlto or kDa
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This reagent is purified
0.97
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Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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Storage
Human ELISAs or ELISA kits will be stored at +4°C. The expiry date is mostly determined by the standard stability. If your ELISA kit is expired we can supply a new standard so it will still be functional.
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Group
Enzyme-Linked Immunosorbent Assay kits
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Description
A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals.
A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
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Gene target
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Short name
OxiSelect™ Nitrotyrosine ELISA test kit (1 )
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Technique
ELISA test, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests.
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Host
antigen assay ELISA kit 1 Kit (96 Wells)
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Alternative name
OxiSelect™ Nitrotyrosine Enzyme-linked immunosorbent assay test reagent (1 plate)
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Alternative technique
elisas, tests, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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