Anti-Mouse CD11b, Biotin (Clone M1/70.15) (rat IgG2b)

• Catalog number
  GEN520037
• Price
  Please ask
• Size
  0.3 miligrams

• Also known as
  CD11b
• Other names
  CD11b; N/A; integrin alpha-M; N/A; N/A
• Category
  Antibodies
• Subcategory
  Mnoclonal antibodies
• Gene name
  CD11b
• Gene name synonims
  N/A
• Other gene names
  ITGAM; N/A; CD11B; N/A
• Clonality
  Monoclonal
• Immunoglobulin isotype
  Rat IgG2b
• Clone
  M1/70.15
• Host organism
  Rat (Rattus norvegicus)
• Species reactivity
  Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
• Specificity and cross reactivity
  CD11b (Mac-1; Ly 40; CR3a) (Mouse CD11b (MAC-1, Ly-40)); Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
• Purification method
  N/A
• Form Appearance
  Biotin (Biotin conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. (Purified from ascitic fluid via Protein G Chromatography))
• Concentration
  Antibody Concentration: 0.1 miligram per 1 mililiter
• Storage and shipping
  Store the antibody at +4 degrees Celsius. For long term storage, aliquot and freeze unused portion the antibody should be stored at -20 degrees Celsius. in volumes appropriate for single usage. Avoid freeze/thaw cycles.
• Tested applications
  Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus, Rattus norvegicus
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

• Gene target
  CD11b   M1/70   IgG2b  
• Short name
  Anti-Mouse CD11b, (Clone M1/70 15) ( IgG2b)
• Technique
  anti-, Mouse, anti, antibody to, antibodies, mouses
• Host
  mouse
• Isotype
  IgG2b
• Label
  Biotin
• Species
  Rat, Mouses, Rats
• Alternative name
  Anti-Anti-Mouse CD11b, Biotin (Clone M1/70.15) (rat IgG2b)
• Alternative technique
  murine, antibodies
• Clone name
  M1

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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