ADPsensor™ Universal Kinase Activity Assay Kit

• Catalog number
  K212-100
• Price
  Please ask
• Size
  100 assays

• Description
  Fast, sensitive, High-throughput compatible, fluorometric Kinase assay with detection limit as low as 1 ng of active Kinase.
• Summary
  Ultrasensitive, Simple, HTP adaptable fluorometric assay kit for Kinase activity measurement. Can detect as low as 1 ng of active Kinase. Detection method- Fluorometric (Ex/Em = 535/587 nm). Applications- Detection of Kinase activity, Characterization of Kinase substrates and inhibitors. Sample Type: Purified/Enriched Kinases, Tissue or cell extracts.
• Detection Method
  Fluorometric (Ex/Em = 535/587 nm)
• Species Reactivity
  Universal
• Applications
  Detection of Kinase activity, Characterization of Kinase substrates and inhibitors.
• Sample Type
  • Purified/semi-purified/immuno-precipitated Kinases • Crude cell/tissue extract
• Features Benefits
  • Ultrasensitive, Simple, HTP adaptable fluorometric assay kit for Kinase activity measurement. • Can detect as low as 1 ng of active Kinase. • Free of ATP interference (up to 1 mM)
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Protein Kinases belong to a large family of phosphotransferases that mediate a wide spectrum of metabolic events via signal transduction pathways. Protein Kinases catalyze transfer of a phosphate group from a phosphate donor to a substrate protein. Kinases modulate the function of numerous proteins and are well-known therapeutic targets in many diseases like cancer, inflammation and diabetes etc. BioVision’s ADPsensor™ Universal Kinase Assay Kit is based on the fluorescence detection of ADP generated by Kinase activity. In this assay, ADP formed during the kinase reaction is converted to an intermediate by the ADP Sensor Mix, which then reacts with a probe to generate a strong and stable fluorescence signal at Ex/Em = 535/587 nm. ATP in the kinase reactions doesn’t interfere with the assay; therefore our kinase assay can be read kinetically (or at endpoint). The kit provides a universal tool to measure kinase activity in presence of substrates of your interest, or to screen/characterize kinase inhibitors. The assay is simple, non-radioactive, highly sensitive (
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  ADPsensor™   Universal   Kinase   Activity   Kit  
• Short name
  ADPsensor™ Universal Kinase Activity Assay Kit
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  ADPsensor™ Universal phosphorylation catalyst Activity test reagent
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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