Vascular Cell Adhesion Molecule-1 Fragment (Western Blot Control) Protein
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Catalog number
OPRB00061
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Price
Please ask
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Size
one vial
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Storage
For short term storage Vascular Cell Adhesion Molecule-1 Fragment (Western Blot Control) Protein may be kep at +4 deg. C. For long term storage keep the aliquoted vials frozen at -20 degrees Celsius.
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Tips
Due to handling and transportation, small amounts of the Vascular Cell Adhesion Molecule-1 Fragment (Western Blot Control) Protein may get caught on the walls or seal of the vials. Prior to use briefly rund the vial(s) through a centrifuge to collect all of the content on the bottom. Avoid freezing and thawing the vials numerous times as such cycles may denaturate the peptide chains and influence the item's performance.
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Description
cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the Vascular Adhesion Molecule-1 ( Blot Control) Protein. For cells, cell lines and tissues in culture till half confluency. Isotype or positive controls by peptides, antibodies and deactivated samples. Whole adhesion and interacting molecules are present in lysates used as reference for ELISA quantification of these molecules and their subunits.
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Test
Western Blot can be performed on tissues and cell extracts to determine the proteins present in your sample after PAGE electrophoresis and transfer on PVDF or Nitrocellulose. PVDF is more advised. The best detection method is using enhanced chemiluminescence or ECL.
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Gene target
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Short name
Vascular Adhesion Molecule-1 ( Blot Control) Protein
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Technique
Western Blot, Control, Blot, western Blots, controls
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Isotype
Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common.
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Alternative name
Vascular Cell Adhesion Molecule-1 Fragment (Western Blot Control) peptide
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Alternative technique
controls, western
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Tissue
cell, control, vascular
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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