CD40Ligand/TRAP, human recombinant
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Catalog number4014-10
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PricePlease ask
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Size10 ug
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SynonymsCD40-L, Tumor necrosis factor ligand superfamily member 5, TNF-related activation protein, TRAP, T cell antigen Gp39, CD154 antigen, sCD40, IGM, IMD3, HIGM1, T-BAM, TNFSF5, hCD40L.
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Alternative_namesCD40-L, Tumor necrosis factor ligand superfamily member 5, TNF-related activation protein, TRAP, T cell antigen Gp39, CD154 antigen, sCD40, IGM, IMD3, HIGM1, T-BAM, TNFSF5, hCD40L.
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DescriptionA protein primarily expressed on activated T cells and involved in immune functions
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RecombinantYes
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SourceE. coli
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Purity by SDS PAGE≥98%
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AssaySDS-PAGE
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Purity≥98%
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Biological activityDetermined by the stimulation of IL-12 induction by human peripheral blood mononuclear cells (PBMC) and the stimulation of IL-8 induction by human PBMC. The expected ED₅₀ for this effect is 5-10 ng/ml. Note: results may vary with PBMC donors.
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Results5-10 ng/ml
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Molecular Weight16.3 kDa
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Storage Temp-20°C
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ShippingGel pack
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Shelf Life12 months
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AppearanceLyophilized protein
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Physical form descriptionLyophilized from 10 mM Sodium Phosphate, pH 7.5
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Reconstitution InstructionsReconstitute in 5-10 mM Sodium Phosphate (pH 7.5) to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers or stored at 4°C for 1 week or –20°C for future use
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Background InformationCD40 Ligand (CD40L/TRAP) is a type II membrane protein that effectuates the helper function of T cells on resting B cells. BioVision’s Human soluble CD40 Ligand is a 16.3 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolACP5, TGFBRAP1, CD40LG, SCYL1
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Short nameCD40Ligand/TRAP, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameCD40Ligand/TRAP, H. sapiens Rec.
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene nameacid phosphatase 5, tartrate resistant
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1989-02-23
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Entrez gene record
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Pubmed identfication
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Classification
- Acid phosphatases
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nametransforming growth factor beta receptor associated protein 1
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Synonyms gene name
- transforming growth factor, beta receptor associated protein 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-02-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CORVET complex
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameCD40 ligand
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Synonyms gene
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Synonyms gene name
- tumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1989-06-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Tumor necrosis factor superfamily
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameSCY1 like pseudokinase 1
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Synonyms gene
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Synonyms gene name
- N-terminal kinase-like
- SCY1-like 1 (S. cerevisiae)
- SCY1-like, kinase-like 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-01-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Armadillo like helical domain containing
- SCY1 like pseudokinases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The number of CD4-POSITIVE T-LYMPHOCYTES per unit volume of BLOOD. Determination requires the use of a fluorescence-activated flow cytometer.
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Tree numbers
- E01.370.225.500.195.107.595.500.150
- E01.370.225.625.107.595.500.150
- E05.200.500.195.107.595.500.150
- E05.200.625.107.595.500.150
- E05.242.195.107.595.500.150
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data