MEC/CCL28, murine recombinant
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Catalog number
7171-10
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Price
Please ask
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Size
10 ug
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Synonyms
Mucosae-associated Epithelial Chemokine, CCL28, CCK-1
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Alternative_names
Mucosae-associated Epithelial Chemokine, CCL28, CCK-1
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Description
Chemoattracts resting CD4, CD8 T cells and eosinophils
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Recombinant
Yes
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Source
E. Coli
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Purity by SDS PAGE
≥98%
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Assay
SDS-PAGE
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Endotoxin Level
< 0.1 ng/ug of protein (
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Activity Specifications test method
Determined by its ability to chemoattract human lymphocytes using a concentration range of 1.0-10.0 ng/ml.
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Biological activity
Determined by its ability to chemoattract human lymphocytes using a concentration range of 1.0-10.0 ng/ml.
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Molecular Weight
12.6 kDa
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized powder
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Physical form description
Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Citrate, pH 4.0.
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Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
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Background Information
MEC is a secreted CC chemokine expressed primarily by epithelial cells of the bronchioles, salivary gland, mammary gland and colon. MEC signals through the CCR10 receptor and chemoattracts resting CD4, CD8 T cells and eosinophils. MEC contains six cysteines including the four highly conserved cysteine residues present in CC chemokines. Recombinant murine MEC is a 12.6 kDa protein containing 111 amino acid residues.
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Amino acid sequence
SEAILPMASS CCTEVSHHVS GRLLERVSSC SIQRADGDCD LAAVILHVKR RRICISPHNR TLKQWMRASE VKKNGRENVC SGKKQPSRKD RKGHTTRKHR TRGTHRHEAS R
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
CCL28, FBXW9
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Short name
MEC/CCL28, murine recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Alternative name
MEC/chemokine (C-C motif) ligand 28, murine Rec.
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Alternative technique
rec
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Alternative to gene target
chemokine (C-C motif) ligand 28, CCL28 and IDBG-19270 and ENSG00000151882 and 56477, chemokine activity, Extracellular, Ccl28 and IDBG-705098 and ENSMUSG00000074715 and 56838, CCL28 and IDBG-629570 and ENSBTAG00000001557 and 535328
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Methods or procedures used to obtain samples of URINE.
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Tree numbers
- E01.370.225.998.762
- E05.200.998.762
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Qualifiers
ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
Product images
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