CaspGLOW™ Fluorescein Active Caspase-8 Staining Kit

Description

Detects activated Caspase-8 by FACS, FL or plate reader
Summary

• Detection method- Fluorescence microscopy, Flow cytometry and fluorescence plate reader (Ex. = 485 nm and Em. = 535 nm) • Species reactivity- Mammalian • Kit size- Convenient sizes (25 and 100 assays) • Applications- Sensitive detection of activated caspase-8 in living cells.
Sample Type

Live cells
Features Benefits

• Simple one-step procedure; takes only 1-2 hours • Fast and convenient • The FITC label allows for direct detection of the activated caspases in apoptotic cells
Storage Conditions

-20°C
Shipping Conditions

gel pack
Shelf life

6-12 months
Background

Activation of caspases plays a central role in apoptosis. The CaspGLOW™ Fluorescein Active Caspase-8 Staining Kit provides a convenient means for sensitive detection of activated caspase-8 in living cells. The assay utilizes the caspase-8 inhibitor, IETD-FMK, conjugated to FITC (FITC-IETD-FMK) as a fluorescent in situ marker. FITC-IETD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-8 in apoptotic cells.
Data Sheet Link
Additional description

Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.

Gene target

CaspGLOW™ Fluorescein Active Caspase-8 Staining Kit
Short name

CaspGLOW™ Fluorescein Active Caspase-8 Staining Kit
Alternative name

CaspGLOW™ Fluorescein functionnal caspase-8 coloring reagent
Alternative technique

kits
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Name

Models, Chemical
Concept

Scope note: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
Tree numbers
- E05.599.495