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Description
Amplite Fluorimetric Goat Anti-Mouse IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence* is a kit that provides an optimized assay protocol that is compatible with HTS liquid handling, as little as 10 pg of a monoclonal antibody/well of a microplate can be detected.It can be used for assays that detect goat anti-mouse IgG as the secondary detection agent.
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Shipping
Blue Ice
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Storage
Freezer
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Shelflife
6 months
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Chemical Components
Component A: Amplite™ Red Peroxidase Substrate ; Component B: H2O2 ; Component C: Assay Buffer; Component D: DMSO; Component E: Goat Anti-Mouse IgG-HRP Conjugate
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Excitation Wavelength
571 nm
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Emission Wavelength
585 nm
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Personal precautions
Use appropriate protective equipment and methods to clean up spilled substances promptly.
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Uses
Laboratory chemicals *For Research Use Only*
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Ordering
To order Amplite Fluorimetric Goat Anti-Mouse IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*, please use the Cat. Nr.11540 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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Tips
Our specialists recommend you to follow carefully the pre-registered instructions for Amplite Fluorimetric Goat Anti-Mouse IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
aat supplies other types of Assays as 1. ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED, Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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Latin name
Capra aegagrus hircus, Mus musculus
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Conjugation
HRP Conjugated, red dye
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Gene target
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Short name
Amplite Fluorimetric Goat Anti-Mouse IgG- Conjugate ELISA Assay Kit *Red *
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Technique
anti-, Assay, ELISA, Mouse, anti, Goat, IgG, antibody to, antibodies, assays, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. goats, IgGs, mouses
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Host
Goat, antigen assay ELISA kit 1 Kit (96 Wells), The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
IgG, IgG
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Label
HRP
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Species
Mouse, Goats, Mouses
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Alternative name
Amplite fluorimetry caprine Antibody toMouse Immunoglobulin G-horseradish peroxidase Conjugate Enzyme-linked immunosorbent assay test reagent *Red Fluorescence*
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Alternative technique
arrays, murine, antibodies, elisas, caprine, kits, immunoglobulins
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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