Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects p70 S6 kinase only when phosphorylated at Thr389.
Shelf time: 12 months in temp.: -20 deg.C
Clonality of antibody:
p70 S6 kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression. P70 S6 kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5’oligopyrimidine tract mRNAs. A second isoform, p85 S6 kinase, is derived from the same gene and is identical to p70 S6 kinase except for 23 extra residues at the N-terminus that encode a nuclear localizing signal. Both isoforms lie on a mitogen-activated signaling pathway downstream of phosphoinositide 3 kinase (PI3K) and the target of rapamycin FRAP/mTOR, and distinct from the Ras/MAP kinase cascade.
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.