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anti-Hsp70 Antibody

  • Catalog number
    3097-100
  • Product name
    anti-Hsp70 Antibody
  • Size
    Hsp70 Antibody
  • Price
    316EUR
  • Supplier
    biovision antibodies-kits-proteins
  • Antibody host:
    Rabbit
  • This antibody reacts with:
    Human, mouse, rat, monkey, hamster, rabbit, bovine
  • Purification method:
    Affinity purified
  • Immunogene sequence:
    Synthetic peptide surrounding amino acid 559 of human Hsp70
  • How to handle this antibody:
    The antibody solution should be gently mixed before use.
  • Form of antibody:
    Liquid
  • This antibody has been succesfully tested for use in following methods:
    Western blot
  • Target of this antibody:
    Hsp70
  • Formulation of this antibody:
    100 µg (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 1% BSA, 0.02% thimerosal.
  • Alternative names of antibody target:
    HSPA4, APG2, APG-2, HSP70RY, HS24/P52, MGC131852 , hsp70RY
  • Recommended usage:
    Western blot analysis (1-2 µg/ml). However, the optimal conditions should be determined individually. The antibody is specific for the inducible form of Hsp70 (Hsp72) and does not cross-react with the constitutive Hsc70 (Hsc73).
  • Storage information:
    Shelf time: 12 months in temp.: -20 deg.C
  • Shipping conditions:
    gel pack
  • Clonality of antibody:
    Polyclonal
  • Isotype:
    Rabbit IgG
  • Background information:
    Hsp70, one class of stress proteins, is comprised of multiple members, all of which bind ATP in vitro, but are localized within different intracellular compartments. These include: a) the constitutive Hsc70 (or cognate) present within the cytosol/nucleus; b) the highly stress-inducible Hsp70 present within the cytosol/ nucleus/nucleolus; c) the constitutive glucose regulated 78 kDa (or BiP) protein present within the lumen of the endoplasmic reticulum; and d) the glucose regulated 75 kDa protein present within the mitochondria. Hsp70 is typically not expressed in the cell under normal growth conditions, but is expressed at high levels in the cell experiencing stress. Consequently, detection of Hsp70 using an antibody specific for the inducible form is quite useful in ascertaining whether a stress response has occurred in the cell. It should be noted, however, that there is a constitutive level of Hsp70 expression in some cell types such as primates, e.g., human and monkey. Nevertheless increased expression of Hsp70 is observed in such cells following stress.
  • Concentration of antibody:
    0.5 mg/ml
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Properties
    If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • Gene target
  • Short name
    anti-Hsp70 Antibody
  • Technique
    Antibody, antibody to, antibodies against human proteins, antibodies for
  • Alternative name
    antibody to-Hsp70 (Antibody to)
  • Alternative technique
    antibodies
1. Gene info
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